2001 Lexus is300 misfire

What is your question?

I have an o1 is300 I recently changed valve cover gasket, spark plugs and wires, I replaced all the vaccum lines on the throttle body, plugged everything back in go to fire the car up and it's misfiring, cylinder 1,3,6 one of the coils was sparking outside of the insulator so I replaced it. Still misfiring, cleaned the maf sensor very well, still misfiring I give up..

What makes this problem better or worse?

Nothing

How long has your 2001 Lexus IS300 had this problem?

A day