engine missfire cylinder 1 on 2000 Chevrolet Venture

idling rough will not accelerate and code from handheld is po301

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2 answers
Check the spark plug for that cylinder ASAP as well as all the ignition wires etc.
I would suggest you being by checking the number 1 cylinder ignition and spark plug and the #1 and #4 spark plug wires. After that diagnosing your engine misfire becomes more difficult.