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Q:

engine missfire cylinder 1 on 2000 Chevrolet Venture

Rookie cbe0621eac06868b3efe0d8d1d3611e23c60d3114864ea2ec19a68cfbd3eebab
idling rough will not accelerate and code from handheld is po301
(2) Answers
Check the spark plug for that cylinder ASAP as well as all the ignition wires etc.
I would suggest you being by checking the number 1 cylinder ignition and spark plug and the #1 and #4 spark plug wires. After that diagnosing your engine misfire becomes more difficult.